Maitake, Hen of the Woods · 2004 · Research Support, Non U.S. Gov'T
High relevance[A method for preparation of genomic DNA from Grifola frondosa and construction of a genomic library].
Grifola frondosa
Key points
- Grifola frondosa, is a valuable medicinal fungus
- High quality total genomic DNA is difficult to prepare due to its high polysaccharide content
- A method for the preparation of Grifola frondosa total genomic DNA and construction of Grifola frondosa, genomic library is described
- Genomic DNA prepared by this method is digested by Sau3A I restriction enzyme
- Constructed genomic library give a titer of 2 x 10(5) transformants/50mg, with a average insert size of 14kb
- This has paved way for the cloning of other Grifola frondosa genes and molecular biology studies
Metadata-grounded summary
Citation abstract
Grifola frondosa, is a valuable medicinal fungus. High quality total genomic DNA is difficult to prepare due to its high polysaccharide content. A method for the preparation of Grifola frondosa total genomic DNA and construction of Grifola frondosa, genomic library is described. Genomic DNA prepared by this method is digested by Sau3A I restriction enzyme. Constructed genomic library give a titer of 2 x 10(5) transformants/50mg, with a average insert size of 14kb. This has paved way for the cloning of other Grifola frondosa genes and molecular biology studies.
Citation
Xu ZX, Cheng D, Li BJ (2004). [A method for preparation of genomic DNA from Grifola frondosa and construction of a genomic library]. Yi chuan = Hereditas PMID: 15640090
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